Liquid Chromatography Systems

Reproducible gradient formation and cutting-edge software for today’s preparative- to production-scale purifications.

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HPLC Systems

Classic small molecule and small protein purification processes utilizing 10 to 15 μm chromatography media will generate backpressures of 70 to 100 bar. Such applications typically require linear gradients to achieve the desired product purity. Our HPLC Systems meet the required pressure rating while offering an array of flexible gradient technology options to fit your needs—whether it is normal phase, reverse-phase or chiral chromatography.

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Gradient Mixing Technologies

We offer a selection of proprietary and patent-pending gradient mixing technologies to permit you to reproducibly separate your target therapeutic at large-scale, whether it be via ion exchange or reverse-phase or normal phase gradient elutions.

Low Pressure Gradient Mixing:

  • Control valves proportion eluents
  • Mixing is on low pressure side of pumps

Classic:

Controller type: Adaptive PAT control or flow control
Eluent supply: Pressurized
Turndown: Up to 30X
Accuracy: ± 2%

Enhanced:

Controller type: Adaptive PAT control or ow control
Eluent supply: Unpressurized/minimally pressurized
Turndown: Up to 90X
Accuracy: ± 2%

High Pressure Gradient Mixing:

  • Pumps proportion eluents
  • Mixing is on high pressure side of pumps

Controller type: Flow control
Eluent supply: Unpressurized/minimally pressurized
Turndown: Up to 90X
Accuracy: ± 2%

Ternary Low Pressure Gradient Mixing:

  • Three control valves proportion eluents
  • Mixing is on low pressure side of pumps
  • Ideal for combination aqueous/organic elutions

Controller type: Adaptive PAT control or ow control
Eluent supply: Pressurized
Turndown: Up to 30X
Accuracy: ± 2%

Industrial, Sanitary or Hygienic Flow Paths

For oligonucleotide or microbial-derived protein purifications that mainly utilize aqueous mobile phases with limited use of organics, or for highly potent active pharmaceutical ingredient (HP-API) purifications where minimized system hold-up is desired, our sanitary or hygienic flow paths are attractive options. Alternatively, our industrial flow path is the most cost-effective for small molecules.

Feature Industrial Sanitary ≤ 0.6 µm Hygienic ≤ 0.5 µm
Eluents Mainly organic solvents Organic solvents, aqueous buffers Organic solvents, aqueous buffers
Process connections Inlets/outlets: TC Inlets/outlets: TC Inlets/outlets: TC
Process connections Internal: NPT & compression Internal: TC or VCR Internal: TC
Wetted MOC 316, 316Ti, 316L 316, 316Ti, 316L 316L
Surface finish spec None SO.6 pm internal sO.S pm internal
Electropolish No Yes, except main pump and ball valves Yes, throughout
Piping Hand welds Orbital welds Orbital welds
Piping documentation Material CofC only Material certs, weld maps, weld logs Material certs, weld maps, weld logs
Low point drains No No Yes
Elastomers PTFE, FFKM, FEP PTFE, FFKM, FEP PTFE, FFKM, FEP
Elastomer certs N/A USP Class VI / BSE-TSE-free USP Class VI / BSE-TSE-free

Software Designed For You

Off-the-shelf chromatography systems may provide familiar software, but have rigid hardware constraints; conversely, systems with customized hardware are typically burdened with cumbersome and dated software. You no longer have to compromise.

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Freedom of Customization

Your unique purification processes or multi-product applications may require an HPLC System that is different than the standard offerings. Our team of artisan engineers and designers will craft you a customized solution based on our standard HPLC System platform.

Features include:

  • Number of inlet ports or fractions
  • Spill-free connections
  • Sample pumps
  • End-of-stream air sensors
  • Pre-filters
  • Sample injection loops
  • Heat exchangers
  • Software customization
  • Hazardous area ratings (NFPA and ATEX)
  • Modified footprints to fit into tight spaces and within fume hoods
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Equipment designed to respect your manufacturing needs

Product Options
Catalog No. Flow Rate Range Pressure Rating Typical DAC Column Pairing
HPC060 6-60 L/h 100 bar 6- 15 cm i.d.
HPC120 12 -120 L/h 100 bar 7.5-20 cm i.d.
HPC180 18-180 L/h 70 bar 10-20 cm i.d.
HPC240 24- 240 L/h 70 bar 10-30 cm i.d.
HPC360 36 – 360 L/h 70 bar 15-40 cm i.d.
HPC600 60 – 600 L/h 70 bar 20-45 cm i.d.
HPC900 90 – 900 L/h 70 bar 30 – 60 cm i.d.
HPC1K2 120- 1200 L/h 70 bar 30-80 cm i.d.
HPC2K4 240- 2400 L/h 70 bar 40- 100 cm i.d.

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