Liquid Chromatography Systems
Reproducible gradient formation and cutting-edge software for today’s preparative- to production-scale purifications.

MPLC Systems
Many mid-sized therapeutics in the 4 to 15 kDa range, such as microbial- derived proteins and oligonucleotides, are purified using 15 to 40 μm chromatography media. Such processes generate higher backpressures than can be accommodated by a typical 6 or 10 bar system but don’t require the pressure rating and upfront capital cost of traditional preparative HPLC. For over a decade, Asahi Kasei Bioprocess has been a leader in 20 bar MPLC Systems for these applications.
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We Can HelpGradient Mixing Technologies
We offer a selection of proprietary and patent-pending gradient mixing technologies to permit you to reproducibly separate your target therapeutic at large-scale, whether it be via ion exchange or reverse-phase gradient elutions.
Low Pressure Gradient Mixing:
- Control valves proportion eluents
- Mixing is on low pressure side of pumps
Classic:
Controller type: Adaptive PAT control or flow control
Eluent supply: Pressurized
Turndown: Up to 30X
Accuracy: ± 2%
Enhanced:
Controller type: Adaptive PAT control or ow control
Eluent supply: Unpressurized/minimally pressurized
Turndown: Up to 90X
Accuracy: ± 2%

High Pressure Gradient Mixing:
- Pumps proportion eluents
- Mixing is on high pressure side of pumps
Controller type: Flow control
Eluent supply: Unpressurized/minimally pressurized
Turndown: Up to 90X
Accuracy: ± 2%

Ternary Low Pressure Gradient Mixing:
- Three control valves proportion eluents
- Mixing is on low pressure side of pumps
- Ideal for combination aqueous/organic elutions
Controller type: Adaptive PAT control or ow control
Eluent supply: Pressurized
Turndown: Up to 30X
Accuracy: ± 2%

Industrial, Sanitary or Hygienic Flow Paths
For oligonucleotide or microbial-derived protein purifications that mainly utilize aqueous mobile phases with limited use of organics, or for highly potent active pharmaceutical ingredient (HP-API) purifications where minimized system hold-up is desired, our sanitary or hygienic flow paths are attractive options. Alternatively, our industrial flow path is the most cost-effective solution.
Feature | Industrial | Sanitary ≤ 0.6 µm | Hygienic ≤ 0.5 µm |
Eluents | Mainly organic solvents | Organic solvents, aqueous buffers | Organic solvents, aqueous buffers |
Process connections | Inlets/outlets: TC | Inlets/outlets: TC | Inlets/outlets: TC |
Process connections | Internal: NPT & compression | Internal: TC or VCR | Internal: TC |
Wetted MOC | 316, 316Ti, 316L | 316, 316Ti, 316L | 316L |
Surface finish spec | None | SO.6 pm internal | sO.S pm internal |
Electropolish | No | Yes, except main pump and ball valves | Yes, throughout |
Piping | Hand welds | Orbital welds | Orbital welds |
Piping documentation | Material CofC only | Material certs, weld maps, weld logs | Material certs, weld maps, weld logs |
Low point drains | No | No | Yes |
Elastomers | PTFE, FFKM, FEP | PTFE, FFKM, FEP | PTFE, FFKM, FEP |
Elastomer certs | N/A | USP Class VI / BSE-TSE-free | USP Class VI / BSE-TSE-free |
Freedom of Customization
Your unique purification processes or multi-product applications may require an MPLC System that is different than the standard offerings. Our team of artisan engineers and designers will craft you a customized solution based on our standard MPLC System platform.
Features include:
- Number of inlet ports or fractions
- Spill-free connections
- Sample pumps
- End-of-stream air sensors
- Pre-filters
- Sample injection loops
- Heat exchangers
- Software customization
- Hazardous area ratings (NFPA and ATEX)
- Modified footprints to fit into tight spaces and within fume hoods

Software Designed For You
Off-the-shelf chromatography systems may provide familiar software, but have rigid hardware constraints; conversely, systems with customized hardware are typically burdened with cumbersome and dated software. You no longer have to compromise.

Equipment designed to respect your manufacturing needs
Product Options
Catalog No. | Flow Rate Range | Pressure Rating | Typical DAC Column Pairing |
MPC180 | 18-180 L/h | 20 bar | 15-20 cmi.d. |
MPC240 | 24-240 L/h | 20 bar | 15-30 cmi.d. |
MPC360 | 36-360 L/h | 20 bar | 15-40 cm i.d. |
MPC600 | 60-600 L/h | 20 bar | 20-45 cm i.d. |
MPC900 | 90-900 L/h | 20 bar | 30-60 cm i.d. |
MPC1K2 | 120-1200 L/h | 20 bar | 30-80 cm i.d. |
MPC2K4 | 240-2400 L/h | 20 bar | 40-100 cm i.d. |