Liquid Chromatography Systems

Reproducible gradient formation and cutting-edge software for today’s preparative- to production-scale purifications.

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MPLC Systems

Many mid-sized therapeutics in the 4 to 15 kDa range, such as microbial- derived proteins and oligonucleotides, are purified using 15 to 40 μm chromatography media. Such processes generate higher backpressures than can be accommodated by a typical 6 or 10 bar system but don’t require the pressure rating and upfront capital cost of traditional preparative HPLC. For over a decade, Asahi Kasei Bioprocess has been a leader in 20 bar MPLC Systems for these applications.

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Gradient Mixing Technologies

We offer a selection of proprietary and patent-pending gradient mixing technologies to permit you to reproducibly separate your target therapeutic at large-scale, whether it be via ion exchange or reverse-phase gradient elutions.

Low Pressure Gradient Mixing:

  • Control valves proportion eluents
  • Mixing is on low pressure side of pumps

Classic:

Controller type: Adaptive PAT control or flow control
Eluent supply: Pressurized
Turndown: Up to 30X
Accuracy: ± 2%

Enhanced:

Controller type: Adaptive PAT control or ow control
Eluent supply: Unpressurized/minimally pressurized
Turndown: Up to 90X
Accuracy: ± 2%

High Pressure Gradient Mixing:

  • Pumps proportion eluents
  • Mixing is on high pressure side of pumps

Controller type: Flow control
Eluent supply: Unpressurized/minimally pressurized
Turndown: Up to 90X
Accuracy: ± 2%

Ternary Low Pressure Gradient Mixing:

  • Three control valves proportion eluents
  • Mixing is on low pressure side of pumps
  • Ideal for combination aqueous/organic elutions

Controller type: Adaptive PAT control or ow control
Eluent supply: Pressurized
Turndown: Up to 30X
Accuracy: ± 2%

Industrial, Sanitary or Hygienic Flow Paths

For oligonucleotide or microbial-derived protein purifications that mainly utilize aqueous mobile phases with limited use of organics, or for highly potent active pharmaceutical ingredient (HP-API) purifications where minimized system hold-up is desired, our sanitary or hygienic flow paths are attractive options. Alternatively, our industrial flow path is the most cost-effective solution.

Feature Industrial Sanitary ≤ 0.6 µm Hygienic ≤ 0.5 µm
Eluents Mainly organic solvents Organic solvents, aqueous buffers Organic solvents, aqueous buffers
Process connections Inlets/outlets: TC Inlets/outlets: TC Inlets/outlets: TC
Process connections Internal: NPT & compression Internal: TC or VCR Internal: TC
Wetted MOC 316, 316Ti, 316L 316, 316Ti, 316L 316L
Surface finish spec None SO.6 pm internal sO.S pm internal
Electropolish No Yes, except main pump and ball valves Yes, throughout
Piping Hand welds Orbital welds Orbital welds
Piping documentation Material CofC only Material certs, weld maps, weld logs Material certs, weld maps, weld logs
Low point drains No No Yes
Elastomers PTFE, FFKM, FEP PTFE, FFKM, FEP PTFE, FFKM, FEP
Elastomer certs N/A USP Class VI / BSE-TSE-free USP Class VI / BSE-TSE-free

Freedom of Customization

Your unique purification processes or multi-product applications may require an MPLC System that is different than the standard offerings. Our team of artisan engineers and designers will craft you a customized solution based on our standard MPLC System platform.

Features include:

  • Number of inlet ports or fractions
  • Spill-free connections
  • Sample pumps
  • End-of-stream air sensors
  • Pre-filters
  • Sample injection loops
  • Heat exchangers
  • Software customization
  • Hazardous area ratings (NFPA and ATEX)
  • Modified footprints to fit into tight spaces and within fume hoods
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Software Designed For You

Off-the-shelf chromatography systems may provide familiar software, but have rigid hardware constraints; conversely, systems with customized hardware are typically burdened with cumbersome and dated software. You no longer have to compromise.

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Equipment designed to respect your manufacturing needs

Product Options
Catalog No. Flow Rate Range Pressure Rating Typical DAC Column Pairing
MPC180 18-180 L/h 20 bar 15-20 cmi.d.
MPC240 24-240 L/h 20 bar 15-30 cmi.d.
MPC360 36-360 L/h 20 bar 15-40 cm i.d.
MPC600 60-600 L/h 20 bar 20-45 cm i.d.
MPC900 90-900 L/h 20 bar 30-60 cm i.d.
MPC1K2 120-1200 L/h 20 bar 30-80 cm i.d.
MPC2K4 240-2400 L/h 20 bar 40-100 cm i.d.

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